The Schmitt Lab is focused on answering the question: How does the cell organize essential processes like metabolism and signaling to coordinate cell function and fate? To answer this question, we develop genetically encoded fluorescent protein-based biosensors to study cellular activity in single cells in real time with high spatial and temporal resolution using live cell fluorescence microscopy. We use these biosensors to (1) study kinase signaling networks which regulate metabolic processes and (2) interrogate sub-cellular dynamics of metabolites. We are interested in understanding how metabolism is spatiotemporally regulated in the cell, and how diseases of metabolic origin, like cancer and inborn errors of metabolism perturb this regulation.